منابع مشابه
The Search for New Antibiotics Targeting the 50S Ribozyme
T o ensure that genetic information is reliably transmitted to daughter cells and converted to protein, cells contain sophisticated molecular machines for replicating DNA, transcribing it into messenger RNA (mRNA), and translating the information encoded in mRNA to make proteins. Each step takes place at a different rate, with DNA polymerase producing new DNA strands at about 1,000 nucleotides ...
متن کاملRibozyme-catalyzed transcription of an active ribozyme.
A critical event in the origin of life is thought to have been the emergence of an RNA molecule capable of replicating a primordial RNA "genome." Here we describe the evolution and engineering of an RNA polymerase ribozyme capable of synthesizing RNAs of up to 95 nucleotides in length. To overcome its sequence dependence, we recombined traits evolved separately in different ribozyme lineages. T...
متن کاملIdentification of gene function and functional pathways by systemic plasmid-based ribozyme targeting in adult mice.
To date, functional genomic studies have been confined to either cell-based assays or germline mutations, using transgenic or knockout animals. However, these approaches are often unable either to recapitulate complex biologic phenotypes, such as tumor metastasis, or to identify the specific genes and functional pathways that produce serious diseases in adult animals. Although the transcription...
متن کاملPhi29 pRNA vector for efficient escort of hammerhead ribozyme targeting survivin in multiple cancer cells.
Ribozymes are potential therapeutic agents which suppress specific genes in disease-affected cells. Ribozymes have high substrate cleavage efficiency, yet their medical application has been hindered by RNA degradation, aberrant cell trafficking, or misfolding when fused to a carrier. In this study, we constructed a chimeric ribozyme escorted by the motor pRNA of bacteriophage phi29 to achieve p...
متن کاملDisruption of glucose sensing and insulin secretion by ribozyme Kir6.2-gene targeting in insulin-secreting cells.
The ATP-sensitive K+ (KATP) channel, composed of Kir6.2 and sulfonylurea receptor (SUR1), in pancreatic beta-cells is believed to serve as a metabolic sensor regulating insulin secretion according to glucose levels. Thus, genetic disruption of Kir6.2 expression may impair KATP channel function in glucose sensing and insulin secretion. Here we show evidence obtained from functional genetic assay...
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ژورنال
عنوان ژورنال: Genome Biology
سال: 2002
ISSN: 1465-6906
DOI: 10.1186/gb-spotlight-20020321-01